FIGURE

Fig. S4

ID

ZDB-FIG-080325-120

Publication

Lachnit et al., 2008 - Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos

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Fig. S4

Zygotic Pou5f1 impact on DEL and EVL vegetal closure progression. (A–B) Z-projection of confocal image stacks of a vegetal view of embryos at approximately 90% epiboly stage stained with Sytox green to visualize nuclei. (A) Wild-type (B) Zspg^m793. (C) Measurement of the distance of opposing DEL and EVL margins. DEL cells of Zspg^m793 displayed an average distance of 341 ± 31 μm and those of wild-type siblings 307 ± 31 μm (p < 0.160) The distance of opposing EVL margins was 220 ± 40 μm in wild-type and 217 ± 19 μm in Zspg^m793 (p < 0.951). Embryos resulted from the cross female spg/+x male spg/spg or from the cross female spg/+x male spg/+. The genotypes of embryos were identified after confocal data acquisition by PCR. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^m793/m793 (AB)
Observed In:	epiboly involved in gastrulation with mouth forming second
Stage:	90%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.