FIGURE

Fig. 7

ID

ZDB-FIG-080325-110

Publication

Lachnit et al., 2008 - Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos

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Fig. 7

Behavior of cells at the ventral margin. (A–D) Live phenotype observations of the ventral leading edge during a time course of 60 min. Membrane tagged GFP/Sytox green labeled embryos were monitored by confocal z-section time lapse analysis during a time course of 1 h starting at 60% epiboly stage. Red arrows mark the EVL leading edge, white arrows the DEL leading edge. (A) Shows the WT leading edge. (B) Shows the MZspg^m793 leading edge. (C) Filopodia formation at the interface between EVL and YSL in a WT embryo (red arrowheads). Δt = 100 s. (D) Filopodia formation between DEL, EVL in WT (white arrowheads). Δt = 250 s. (A, B) Scale bar: 100 μm. (C, D) Scale bar: 50 μm (see also Movies S3, S4, and S5).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^m793/m793 (AB)
Observed In:	epiboly involved in gastrulation with mouth forming second
Stage Range:	Shield to 75%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.