FIGURE

Fig. S3

ID

ZDB-FIG-080325-117

Publication

Lachnit et al., 2008 - Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos

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Fig. S3

Maternal Pou5f1 impact on the DEL and EVL phenotype. (A–C) Z-projection of confocal image stacks of 85% epiboly embryos stained with Sytox green to visualize nuclei. (A) WT; (B) Mspg^m793; (C) MZspg^m793. A delay of EVL and DEL is detectable based on altered distribution of nuclei. (D–F) Higher magnification of the EVL margin of confocal z-projections of Alexa488-Phallodin stained embryos. (D) WT; (E) Mspg^m793; (F) MZ spg^m793. Note delay in change of marginal EVL cell shape and reduced stain intensity of the punctate F-Actin band in the YSL. yc: yolk cell. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^m793/m793 (AB) pou5f3^m793/+ (AB)
Observed In:	cell morphogenesis epiboly involved in gastrulation with mouth forming second EVL blastoderm cell yolk syncytial layer actin filament
Stage Range:	50%-epiboly to 75%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.