Fig. 6

Non-cell autonomous delay of DEL cell migration in MZspg embryos. Confocal z-stack time lapse recordings of mosaic embryos, starting at shield stage for 60 min. An approximately equal amount of cells from WT and MZspg^m793 donors labeled with different fluorescent dyes were collected and co-transplanted into the ventral margin of a non-labeled host. Donors were either labeled with Alexa488-dextran (MW: 10 kDa) or Alexa546-dextran (MW: 10 kDa). (A, B) Cells transplanted into a WT host. (D, E) Cells transplanted into a MZspg^m793 host. (B, E) Tracking lines of transplanted WT cells. (B′, E′) Tracking lines of transplanted MZspg^m793 cells at the end of the time lapse. (C, F) Measurements of the net velocity of 10–20 transplanted cells. Velocity was normalized on WT velocity and depicted as % of WT data including standard deviation. (C) Net velocity of cells in a WT environment (n = 4): WT = 100 ± 12%; MZspg^m793 = 95 ± 13%; p = 0.828. (F) Net velocity of cells in a pou5f1 deficient environment (n = 2): WT = 100 ± 22%; MZspg^m793 = 110 ± 18%; p = 0.825. No significant difference in velocity was observed. Scale bar: 46.2 μm (Movies S1 and S2).