FIGURE

Fig. 2

ID

ZDB-FIG-241203-2

Publication

Volkov et al., 2024 - Samd7 represses short-wavelength cone genes to preserve long-wavelength cone and rod photoreceptor identity

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Fig. 2

Rod- and green cone-specific gene expression is reduced, and blue and UV-cone-specific gene expression is increased in samd7?/? eyes. (A) Volcano plot of bulk RNA-seq data from samd7?/? vs. WT 5-dpf eyes demonstrates a marked reduction in the expression of red/green-cone-specific arr3a and green-cone-specific opsins opn1mw1 and opn1mw2. (B) A magnified view of the dotted portion of panel A demonstrates a marked reduction in the expression of multiple rod-specific genes, a subset of which are annotated. The expression of multiple blue and UV cone-specific genes is increased in the mutant, including blue/UV-cone-specific arr3b and blue opsin (opn1sw2). UV opsin (opn1sw1) appeared somewhat increased, but the change is not statistically significant (p-adj = 0.07, Dataset S1). Among red-cone-specific genes, si:busm1-57f23.1 is decreased, and opn1lw1 is increased. Exorh, which is normally restricted to a subset of pineal photoreceptors, is also increased (42). (C) Bar plots of opsin expression (DESeq2-normalized counts) from RNA-seq data in panels A and B (mean � SD; n = 3 per group) *p-adj < 0.05. ns, not significant. SD, standard deviation.

Expression Data

Genes:	arr3a arr3b exorh foxq2 gnat1 gngt1 nr2e3 opn1lw1 opn1lw2 opn1mw1 opn1mw2 opn1sw1 opn1sw2 rho rom1a rom1b saga sagb samd7 skor1a tbx2a
Fish:	stl887Tg/stl887Tg
Anatomical Term:	eye
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stl887Tg/stl887Tg
Observed In:	eye
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA