ZFIN Image: Volkov et al., 2024, Fig. 2

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Figure Caption

Fig. 2 Rod- and green cone-specific gene expression is reduced, and blue and UV-cone-specific gene expression is increased in samd7?/? eyes. (A) Volcano plot of bulk RNA-seq data from samd7?/? vs. WT 5-dpf eyes demonstrates a marked reduction in the expression of red/green-cone-specific arr3a and green-cone-specific opsins opn1mw1 and opn1mw2. (B) A magnified view of the dotted portion of panel A demonstrates a marked reduction in the expression of multiple rod-specific genes, a subset of which are annotated. The expression of multiple blue and UV cone-specific genes is increased in the mutant, including blue/UV-cone-specific arr3b and blue opsin (opn1sw2). UV opsin (opn1sw1) appeared somewhat increased, but the change is not statistically significant (p-adj = 0.07, Dataset S1). Among red-cone-specific genes, si:busm1-57f23.1 is decreased, and opn1lw1 is increased. Exorh, which is normally restricted to a subset of pineal photoreceptors, is also increased (42). (C) Bar plots of opsin expression (DESeq2-normalized counts) from RNA-seq data in panels A and B (mean � SD; n = 3 per group) *p-adj < 0.05. ns, not significant. SD, standard deviation.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA