Fig. 6

ABCA7 regulates synaptic integrity and astroglial proliferation through BDNF (A) Experimental setup for investigating the biological relevance of NPY to abca7 function and amyloid-induced alterations in synaptic integrity, microglial activity, and astroglial proliferation. (B) Immunofluorescence for SV2 + L-plastin and GS + PCNA with DAPI counterstains in abca7+/? + PBS, abca7+/? + A?42, abca7+/? + NPY, and abca7+/? + A?42 + NPY brains. (C) Quantification for number of SV2-positive synaptic puncta, L-plastin-positive microglia, and GS/PCNA double-positive proliferating astroglia. n = 4 animals from both sexes and ?20 brain sections per group. One-way ANOVA with Tukey?s multiple comparison test was used for statistical analyses. No comparison bar indicates no significance. (D) BDNF immunoreactivity in wild-type and abca7 knockout zebrafish with and without A?42. A?42 induced BDNF (red) in wild-type animals but not in abca7 knockout. Injection of NPY with or without A?42 restores the induced BDNF expression levels. Non-parametric Kolmogorov-Smirnov test comparing cumulative distributions and one-way ANOVA with Tukey?s multiple comparison test were used for statistical analyses. (E) Immunofluorescence for SV2 and L-plastin with DAPI counterstain in abca7+/? + A?42 + PBS and abca7+/? + A?42 + BDNF zebrafish brains. Quantification for the number of SV2-positive synaptic puncta and the number of L-plastin-positive microglia. Non-parametric Kolmogorov-Smirnov test comparing cumulative distributions was used for statistical analyses. BDNF rescues synaptic integrity changes in abca7+/? knockout similar to NPY. n = 4 animals from both sexes, with 18 brain sections per group. ?p < 0.0332, ??p < 0.0021, ???p < 0.0002, ????p < 0.0001; not significant (ns), p > 0.0332. Scale bars, 50 ?m. See also Figures S1 and S4 , Data S1 and S3 , and Table S1 .