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Expression of zebrafish tribbles are located in the brain in larvae and in immune cell subpopulations in adults. (A) Whole-mount in situ hybridisation of the pan-leukocyte marker L-plastin was used as a positive control to stain leukocytes, present predominantly in the caudal hematopoietic tissue (CHT). Scale bar = 200 µm. (B) Representative images of antisense and sense (control) whole-mount in situ hybridisation of tribbles1. Numbers correspond to number of fish with depictured pattern out of total fish imaged. N = 19–20 fish. (C) Antisense and sense (control) whole-mount in situ hybridisation of tribbles2. N = 17–22 fish. (D) Antisense and sense (control) whole-mount in situ hybridisation of tribbles3. N = 17–20 fish. (E) Magnified dorsal views (×8 magnification) showing distinct expression of each trib isoform in the brain (representative images from each group shown). Scale bar = 200 µm. (F–H) Gene expression of adult zebrafish leukocytes determined using the Zebrafish Blood Atlas (Athanasiadis et al., 2017). Each point represents a separate scRNAseq sample (cell); replicates performed across multiple zebrafish wildtype and transgenic strains. Each arm of schematic indicates a separate blood cell population (labelled). Deeper colour indicates higher expression (log10 scale bars described for each gene).
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