Figure 4—figure supplement 1.

tribbles RNA overexpression caused no observable developmental defects.

(A) cDNA sequences of zebrafish trib isoforms (trib1, trib2, and trib3) were cloned into the expression plasmid pCS2+. (B) mRNA of trib isoforms (100 ng/μl) was synthesised from pCS2+ plasmids and 1 nl was injected into one-cell stage zebrafish embryos, which resulted in healthy development. Larvae imaged at 2 dpf using stereoscope at ×4 magnification (head images at ×8 magnification). Scale bars = 100 µM.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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