Rescue of motor behavior deficits by the addition of human wild-type TUBA4A mRNA. Zebrafish embryos were subjected to a touch-evoked escape response (TEER) assay at 48 hpf after injection with an ATG morpholino against zebrafish tuba8l2 (0.160 mM) with or without the injection of wild-type human TUBA4A mRNA (300 ng/μl). Non-injected embryos were also included in the analysis. (a) Total distance for AMO 0.160 mM + TUBA4A mRNA compared to non-injected (non-significant) and AMO 0.160 mM condition (p < 0.0001), and non-injected (p < 0.0001) compared to AMO 0.160 mM condition. (b) Average velocity for AMO 0.160 mM + TUBA4A mRNA compared to non-injected (p = 0.0018) and AMO 0.160 mM condition (p < 0.0001), and non-injected (p < 0.0001) compared to AMO 0.160 mM condition. (c) Maximal instant velocity for AMO 0.160 mM + TUBA4A mRNA compared to non-injected (p = 0.0110) and AMO 0.160 mM condition (p < 0.0001), and non-injected (p < 0.0001) compared to AMO 0.160 mM condition. Kruskal-Wallis test with Dunn’s multiple comparisons; N = 3 experiments; n = 10–15 zebrafish embryos per group per experiment; which each data point representing an individual zebrafish embryo. (d) Representative escape traces from 10 zebrafish embryos per group shown as a visual example. *p < 0.05, **p < 0.01, and ****p < 0.0001. AMO, morpholino; hpf, hours post fertilization.
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