FIGURE

FIGURE 6

ID
ZDB-FIG-240311-11
Publication
Van Schoor et al., 2024 - TUBA4A downregulation as observed in ALS post-mortem motor cortex causes ALS-related abnormalities in zebrafish
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FIGURE 6

Zebrafish microtubule polymerization is not affected by tuba8l2 knockdown. At 48 hpf, an EB3 comet assay was performed in single CaP motor neurons after co-injection of an ATG morpholino against tuba8l2 (0.160 mM) or a control morpholino (0.160 mM). (a–d) Quantification of the comet run metrics extracted from kymograms show no difference in microtubule growth kinetics. (a) Average comet distance for AMO 0.160 mM (p = 0.8230) compared to AMO control condition. (b) Average comet duration for AMO 0.160 mM (p = 0.7340) compared to AMO control condition. (c) Average comet velocity for AMO 0.160 mM (p = 0.4605) compared to AMO control condition. (d) Average comet density for AMO 0.160 mM (p = 0.3741). Unpaired t-test (a) or Mann-Whitney test (b,c,d); N = 3 experiments; n = 20–37 zebrafish embryos with each data point representing an individual zebrafish embryo. (e) Representative image (composite z-stack projection) of EB3-GFP in AMO control zebrafish embryo. Red box: representative area for the comet analysis in a distal segment in the CaP. (f) Representative kymograms represent microtubule growth extracted through time-lapse imaging (500 ms/5 min) of EB3-GFP comets in single CaP motor neurons distal arbors at 48 hpf. Each pixel on the Y-axis represents one timepoint image (time) projected against the neurite length (distance) on the X-axis. AMO, morpholino; hpf, hours post fertilization; CaP, caudal primary; scale bar, 25 μm; t, time (5 min); d, distance (average: 17 μm segments).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.