|
Specific tuba8l2 knockdown in zebrafish induces changes in post-translational modifications of tubulin. (a–f) Western blot was performed on whole fish lysate from 48 hpf embryos injected with ATG morpholino against tuba8l2 (0.160 mM) vs. control morpholino (0.160 mM). N = 6 experiments; n = 10–30 zebrafish per group per experiment. (a–c) Representative western blot for acetylated α-tubulin (a), detyrosinated α-tubulin (b) and polyglutamylated tubulin (c). (d–f) Quantification of acetylated α-tubulin (d) (p = 0017), detyrosinated α-tubulin (e) (p < 0.0001) and polyglutamylated tubulin (f) (p = 0.2014) protein levels relative to α-tubulin after normalization to GAPDH for the different injection conditions. Data represent mean ± SEM. Unpaired t-test: ** p < 0.01, *** p < 0.001. AMO, morpholino; hpf, hours post fertilization; ctrl, control.
|
