ZFIN Image: Van Schoor et al., 2024, FIGURE 5

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FIGURE 5

Rescue of motor behavior deficits by the addition of human wild-type TUBA4A mRNA. Zebrafish embryos were subjected to a touch-evoked escape response (TEER) assay at 48 hpf after injection with an ATG morpholino against zebrafish tuba8l2 (0.160 mM) with or without the injection of wild-type human TUBA4A mRNA (300 ng/μl). Non-injected embryos were also included in the analysis. (a) Total distance for AMO 0.160 mM + TUBA4A mRNA compared to non-injected (non-significant) and AMO 0.160 mM condition (p < 0.0001), and non-injected (p < 0.0001) compared to AMO 0.160 mM condition. (b) Average velocity for AMO 0.160 mM + TUBA4A mRNA compared to non-injected (p = 0.0018) and AMO 0.160 mM condition (p < 0.0001), and non-injected (p < 0.0001) compared to AMO 0.160 mM condition. (c) Maximal instant velocity for AMO 0.160 mM + TUBA4A mRNA compared to non-injected (p = 0.0110) and AMO 0.160 mM condition (p < 0.0001), and non-injected (p < 0.0001) compared to AMO 0.160 mM condition. Kruskal-Wallis test with Dunn’s multiple comparisons; N = 3 experiments; n = 10–15 zebrafish embryos per group per experiment; which each data point representing an individual zebrafish embryo. (d) Representative escape traces from 10 zebrafish embryos per group shown as a visual example. *p < 0.05, **p < 0.01, and ****p < 0.0001. AMO, morpholino; hpf, hours post fertilization.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.