Fig. 7

A Representative microscopy images of Tg(lck:IRF4;p53^wt/wt) transgenic fish treated with vehicle (DMSO; n = 5) or JQ1 (4 μM; n = 5). Panels show merged fluorescence and brightfield images. Scale bar = 4 mm. B Quantification of tumor volume for five independent animals on days 1, 2, 5, 7, 8, 12 and 15 compared to that on day 0. Integrated fluorescence intensity was quantified by ImageJ (NIH) software and normalized to body size; *p < 0.05, **p < 0.01, and ***p < 0.001 by two-tailed unpaired Student’s t-tests. Bars indicate mean and SD values. Day 1 p = 0.07; day 2 p = 0.83; day 5 p = 0.04; day 7 p = 7.7 × 10⁻⁵; day 8 p = 0.001; day 12 p = 1 × 10⁻⁵; day 15 p = 1.7 × 10⁻⁵. C Gene set enrichment analysis plots showing the overall pattern of gene expression changes of Group I + II genes upon JQ1 treatment. Each solid bar represents one gene within the gene set. The normalized enrichment scores (NES) by dataset permutations and p-values are indicated. D Bar charts showing the expression level of seven selected genes in control and JQ1-treated samples (n = 3 independent animals for each group). The data represent the mean ± SEM (shown as bars) of replicate samples; *p < 0.05 and **p < 0.01 by two-tailed unpaired Student’s t-tests. Source data are provided as a Source Data file.