FIGURE

Fig. 1

ID

ZDB-FIG-220509-11

Publication

Amanda et al., 2022 - IRF4 drives clonal evolution and lineage choice in a zebrafish model of T-cell lymphoma

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Fig. 1

Overexpression of wild-type <italic>IRF4</italic> induces tumors in zebrafish.

A Schematic diagram of the experimental procedure used to overexpress the human IRF4 gene under the zebrafish lck promoter. B Tumor onset in F0 animals was analyzed in the control Tg(lck:mCherry) (n = 7) and Tg(lck:IRF4) transgenic (n = 21) groups; p = 0.007 according to the Gehan-Breslow-Wilcoxon test. C Tumor onset in F1 animals was analyzed in the control Tg(lck:mCherry;p53^wt/wt) (n = 30), Tg(lck:mCherry;p53^wt/mut) (n = 25), Tg(lck:IRF4;p53^wt/wt) (n = 79), Tg(lck:IRF4;p53^wt/mut) (n = 32) and Tg(lck:IRF4;p53^mut/mut) (n = 21) groups. p = 0.0052 for Tg(lck:mCherry;p53^wt/wt) or Tg(lck:mCherry;p53^wt/mut) vs Tg(lck:IRF4;p53^wt/wt); p < 0.0001 for Tg(lck:IRF4;p53^wt/wt) vs Tg(lck:IRF4;p53^wt/mut); and p < 0.0001 for Tg(lck:IRF4;p53^wt/wt) vs Tg(lck:IRF4;p53^mut/mut) according to the Gehan-Breslow-Wilcoxon test. D Representative microscopy images of F1 animals: control Tg(lck:mCherry;p53^wt/wt), Tg(lck:IRF4;p53^wt/wt), Tg(lck:IRF4;p53^wt/mut) and Tg(lck:IRF4;p53^mut/mut) zebrafish at 8, 16, 24, and 32 weeks postfertilization. Panels show merged fluorescence and brightfield images. Scale bar = 4 mm. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ns106Tg/ns106Tg tp53^zdf1/+; ns106Tg/ns106Tg tp53^zdf1/zdf1; ns106Tg/ns106Tg
Observed In:	lymphocyte neoplasm
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.