FIGURE

Fig. 1

ID

ZDB-FIG-210428-94

Publication

Xia et al., 2021 - A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish

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Fig. 1

Single-cell transcriptome map of zebrafish CHT. (A) A schematic paradigm of tissue processing and fluorescence-activated cell sorting for scRNA-seq profiling of CHT. ECs, HCs, and NCs were sorted from Tg (kdrl:mCherry/CD41:GFP) embryos across three developmental stages (55 hpf, 3.5 dpf, and 4.5 dpf). (B) UMAP plot showing 23 CHT cell clusters across three developmental stages (28,777 cells). Cells are colored by their cell-type annotation and numbered according to the legend beside. (C) Heatmap showing blocks of DEGs (top 10 genes) in HSPCs, neural cells, erythrocytes, myeloid cells, ECs, epithelial cells, epidermis, fibroblasts, and muscle cells in the CHT. (D) UMAP visualization of the expression of curated feature genes for cell-type identification.

Expression Data

Genes:	EGFP elavl3 fli1 hbae3 hdr hemgn ikzf1 mCherry mfap4.1 mmp13a mpx myb snap25a spi1b tuba1c vegfc
Fish:	la2Tg; s896Tg
Anatomical Terms:	caudal hematopoietic tissue endothelial cell caudal hematopoietic tissue hematopoietic stem cell caudal hematopoietic tissue myeloid cell caudal hematopoietic tissue nerve caudal hematopoietic tissue nucleate erythrocyte endothelial cell hematopoietic cell
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA