lpin1 knockdown affects gene expressions in the muscle and neuron development of zebrafish larva. The expression of somite marker deltaC in control embryos (A) and lpin1 morphant (A') embryos at 8-somite stage. Red arrows indicate newly formed somites and red bars indicate the length of presomitic mesoderm. Control embryos (B) and lpin1 morphant (B') embryos at 25 hpf were stained for actn3a. Note the shape of myotome, indicated by red angle, was changed in lpin1 morphants. The expression of neuron markers neurog1(C, C') at 50 hpf and isl1(D, D') at 25 hpf in control embryos (C, D) and lpin1 morphants (C', D'). In lpin1 morphants, the expression of neurog1 was reduced in the midbrain (mb), hindbrain (hb), and spinal cord (sp) (pink arrows); and that of islet1 was reduced in PMNs (yellow arrows). Scale bar: 100 μm. The expressions of muscle (E) and neuron (F) markers, as determined by qRT-PCR, were significantly changed in lpin1 morphants at 4 dpf and 1 dpf respectively. Lpin1 siRNA knockdown in C2C12 (G) and U87 cells (H) resulted in abnormal expressions of muscle and neuron markers. Values in (E, F, G and H) represent means ± SE of data from three independent experiments, *p< 0.05, **p< 0.01, and ***p< 0.001 (Student's t-test).
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