High-fat diets induced hepatic steatosis in adult and larval zebrafish. a Body weight of adult zebrafish fed with a control diet or high-fat diet (HFD) for 4 weeks. b Representative image of the abdominal subcutaneous fatty tissue histology with H&E staining. The scale bar is 100 μm. cTriacylglyceride (TAG) content in liver (n = 3, pool of three zebrafish per sample). dRepresentative liver histology image by H&E staining. The scale bar is 50 μm. e The expression of lipid metabolism-related genes in liver (n = 5, pool of three zebrafish per sample). f Representative image of whole-mount oil red O staining and H&E staining of liver sections in larvae fed control diet and high-fat diet. The scale bar is 100 μm. g The expression of lipid metabolism-related genes in zebrafish larvae. Gene expression was analyzed using cDNA prepared from pools of larvae in each group after seven days of feeding (n = 3, pool of 20 larvae per sample). Significance was established using a two-tailed student’s t-test. Differences are considered significant at P < 0.05 (*) and P < 0.01 (**)
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