FIGURE

Fig. S1

ID

ZDB-FIG-190611-33

Publication

Turner et al., 2019 - Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits

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Fig. S1

(A-A’) Analysis of live 50hpf Control (A) and Morpholino injected embryos (A’) show that the morphological dent caudal to the MHB (white arrow), indentations in the retina (black arrow) and coloboma are successfully phenocopied.

(B) Electrophoresis gel of RT-PCR analysis confirmed that several missplicing events occur as at least five variably sized products were generated (++ lane).

(C-C’) Injection of degradation-resistant slbp^TT-AA-RFP synthetic RNA into WT(C) and ele(C’)mutants rescues ele phenotype. Note heart oedema still present in some cases (C’).

(D-D’) Frontal sections of 3dpf wildtype (D) and slbp^ty77e (D’) retinas showing anti-γ-tubulin labelled neurites/neuropil (magenta) and Rho4D2-expressing rod photoreceptors (green).

(TIF)

Expression Data

Antibodies:	Ab1-tubg1 Ab5-rho
Fish:	WT slbp^ty77e/ty77e
Anatomical Terms:	eye neuron projection eye photoreceptor cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slbp^ty77e/ty77e WT + MO1-slbp
Knockdown Reagent:	MO1-slbp
Observed In:	closure of optic fissure eye neuron projection eye photoreceptor cell midbrain hindbrain boundary posterior side optic fissure
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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