FIGURE

Fig. 3

ID

ZDB-FIG-190611-29

Publication

Turner et al., 2019 - Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits

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Fig. 3

Slbp is required cell autonomously for retinal neuron differentiation.

(A, A’) Images from live timelapse recordings of Tg(atoh7:GFP)^rw021 GFP-transgene expression in retinal neurons in control (A) and slbp^ty77e (A’) eyes at 30hpf. (B-B’) Frontal sections of 3dpf wildtype (B) and slbp^ty77e (B’) retinas showing anti-γ-tubulin labelled neurites/neuropil (magenta) and zpr1-expressing cone photoreceptors (green). The arrowhead points to a few remaining zpr1+ cone cells in the slbp^ty77e mutant eye. (C-C’) Frontal sections of 3dpf wildtype (wt) host retinas containing transplanted wildtype (C) or slbp^ty77e mutant (C’) GFP-labelled cells (green).(D-D’) High magnification images of transplanted cells in C-C’. Note the break in the plexiform layer in the retina (arrowhead) in the vicinity of the slbp^ty77e mutant cells. Abbreviations: nr, nasal retina. Scale bars: (A-C’) 50μm; (D-D’) 25μm.

Expression Data

Gene:	GFP
Antibodies:	Ab1-tubg1 zpr-1
Fish:	WT slbp^ty77e/ty77e rw021Tg slbp^ty77e/ty77e; rw021Tg
Anatomical Terms:	eye neuron projection retinal cone cell retinal ganglion cell
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slbp^ty77e/ty77e slbp^ty77e/ty77e; rw021Tg
Observed In:	eye neuron projection retinal cone cell retinal ganglion cell
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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