ZFIN Image: Ghosh et al., 2018, Fig. 3

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Fig. 3

Expression of the r5/r6 gene-set is dependent on hnf1ba and valentino. Expression of r5/r6 candidate genes was assayed via ISH in (i) WT, (ii) hoxb1b mutant, (iii) hoxb1a mutant, (iv) hnf1ba mutant and (v) valentino mutant zebrafish lines. The genes assayed include a tox3, b sema3fb, c mpz, d gas6, e celf2, f nr2f2, g col15a1b and h gbx1. krox20 (red), which is expressed in r3 and r5, was used to position the expression domains of several genes as indicated. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 12hpf, 14hpf, 16hpf and 19hpf were imaged as whole-mounts. 24hpf embryos were flat-mounted for imaging. Black brackets mark the smaller r5 domain. Red bracket in (Hiii) indicates expression of gbx1 throughout the hindbrain, as a result of reappearance of gbx1 expression in r4 of hoxb1a mutants

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Acknowledgments

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