FIGURE

Fig. 7

ID

ZDB-FIG-170825-38

Publication

Wehner et al., 2017 - Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish

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Fig. 7

Overexpression of col12a1a promotes axon regeneration in Wnt/β-catenin pathway-inhibited and wild type zebrafish larvae. a cerulean mRNA is robustly detectable 24 h after the last heat shock in hs:col12a1a-p2a-Cerulean transgenic larvae. b col12a1a overexpression increases anti-Col XII immunoreactivity in the lesion site of Wnt/β-pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (arrow; t-test: ***P < 0.001). c col12a1a overexpression rescues axon regeneration in Wnt/β-catenin pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (Fischer’s exact test: ***P < 0.001). d col12a1a overexpression improves functional recovery in Wnt/β-catenin pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (t-test: ***P < 0.001). e col12a1a overexpression is sufficient to augment axon regeneration (Fischer’s exact test: *** P< 0.001). a–e Views are lateral (dorsal is up, rostral is left). BF: brightfield. Scale bars: 200 µm c and 100 µm a–c, e. Error bars indicate s.e.m

Expression Data

Genes:	Cerulean DsRed
Fish:	ue104Tg zf148Tg ue104Tg; zf148Tg
Conditions:	heat shock chemical treatment by environment: IWR-1-endo, heat shock, perforation: spinal cord
Anatomical Terms:	regenerating tissue axon whole organism
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ue104Tg ue104Tg; zf148Tg
Conditions:	heat shock chemical treatment by environment: IWR-1-endo, heat shock, perforation: spinal cord
Observed In:	spinal cord axon regeneration swimming whole organism
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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