ZFIN Image: Wehner et al., 2017, Fig. 7

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Figure Caption

Fig. 7

Overexpression of col12a1a promotes axon regeneration in Wnt/β-catenin pathway-inhibited and wild type zebrafish larvae. a cerulean mRNA is robustly detectable 24 h after the last heat shock in hs:col12a1a-p2a-Cerulean transgenic larvae. b col12a1a overexpression increases anti-Col XII immunoreactivity in the lesion site of Wnt/β-pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (arrow; t-test: ***P < 0.001). c col12a1a overexpression rescues axon regeneration in Wnt/β-catenin pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (Fischer’s exact test: ***P < 0.001). d col12a1a overexpression improves functional recovery in Wnt/β-catenin pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (t-test: ***P < 0.001). e col12a1a overexpression is sufficient to augment axon regeneration (Fischer’s exact test: *** P< 0.001). a–e Views are lateral (dorsal is up, rostral is left). BF: brightfield. Scale bars: 200 µm c and 100 µm a–c, e. Error bars indicate s.e.m

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Acknowledgments

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