Fig. S3

Wnt/β-catenin pathway is upregulated in basal keratinocytes and fibroblast-like cells but not muscle or immune cells after a lesion.

(a-b) Subepidermal 6xTCF:dGFP⁺/p63⁻ cells (arrows) do not co-label with MyHC antibodies, a marker for slow twitch muscle cells. Arrowheads indicate 6xTCF:dGFP⁺/p63⁺ basal keratinocytes. Note that close to the disorganized lesion center some subepidermal 6xTCF:dGFP⁺/p63⁺ cells are found sandwiched between p63⁺ basal keratinocytes and MyHC⁺ slow twitch muscle fibers (insets), which represents a typical dermal fibroblast location in zebrafish larvae.

(c) 6xTCF:dGFP⁺ cells do not co-label with L-Plastin, a marker for innate immune cells.

(d) The 6xTCF:dGFP Wnt reporter is active in p63⁺ basal keratinocytes (arrowheads) and in subepidermal p63⁺ cells (arrows) in the lesion site.

(e) Subepidermal cells (below dashed line) co-express col1a2 (red) and gfp mRNA (green) in lesioned 6xTCF:dGFP transgenic animals (arrow). Dashed line indicates junction between basal epithelium and subadjacent connective tissue.

(f-g) Subepidermal cells (below dashed line) co-express fn1a (red) and gfp mRNA (green) in lesioned 6xTCF:dGFP transgenic animals (arrow). Dashed line indicates junction between basal epithelium and subadjacent connective tissue.

(h) Close to the disorganized lesion center, some 6xTCF:dGFP⁺ cells are found to cover the spinal cord (arrows), which represents a typical meningeal fibroblast location. Dashed line indicates spinal cord.

(a-h) Views are transversal (dorsal is up). Scale bars: 100 μm (a-c) and 50 μm (d-h).