FIGURE

Fig. S9

ID

ZDB-FIG-170828-6

Publication

Wehner et al., 2017 - Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S9

Wnt/β-catenin signaling controls Col XII matrix deposition in a spinal lesion site through which regenerating axons grow.

(a) Anti-Col XII immunoreactivity is increased in the lesion site (arrows) at 1 dpl and 2 dpl compared to adjacent unlesioned tissue and to unlesioned animals at the same trunk position.

(b) Inhibition of Wnt/β-catenin signaling via heat shock-induced systemic overexpression of the pathway antagonist dkk1 interferes with Col XII deposition in the lesion site, as determined by quantification of lesion site immunoreactivity (t-test: **P<0.01).

(c) Double immunolabelling of axons (anti-acetylated Tubulin⁺) and Col XII shows little to no Col XII deposition in the lesion site at 0.5 dpl (12 hpl) and axon are yet to enter the lesion site (asterisk). At 1 dpl, anti-Col XII immunoreactivity is markedly increased in the lesion site and axon have entered the Col XII-rich lesion site (arrow). Confocal depth was limited to spinal cord. Note, that the 1 dpl dataset is the same as presented in Fig. 4f.

(a-c) Views are lateral (dorsal is up, rostral is left). BF: brightfield. Scale bars: whole mounts, 100 μm (a-b) and 50 μm (c). Error bars indicate s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.