FIGURE

Fig. 5

ID

ZDB-FIG-170825-36

Publication

Wehner et al., 2017 - Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish

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Fig. 5

Knockdown of col12a1a/b inhibits functional axon regeneration. a Cartoon showing strategy used for systemic delivery of Vivo-Morpholino (MO), which acts as cell-permeable translation blocking anti-sense molecules. MOs against col12a1a and col12a1b were co-injected into the cardiac ventricle at 3 dpf, 4 h before lesion. b col12a1a/b MO injection strongly reduces Col XII immunoreactivity in the lesion site compared to control MO injected animals. (t-test: ***P < 0.001). c col12a1a/b MO injection inhibits axon regeneration after lesion (Fischer’s exact test: ***P < 0.001). d col12a1a/b MO injection interferes with functional recovery after lesion (two-way ANOVA: F_1,116 = 5.534, p < 0.05; t-test: ***P < 0.001). a–d Lateral views are shown (dorsal is up, rostral is left). BF: brightfield. n.s.: not significant. Scale bars: 200 µm c and 100 µm (b,c). Error bars indicate s.e.m

Expression Data

Gene:	DsRed
Fish:	zf148Tg zf148Tg + MO1-col12a1a + MO1-col12a1b
Condition:	perforation: spinal cord
Knockdown Reagents:	MO1-col12a1a MO1-col12a1b
Anatomical Term:	regenerating tissue axon
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf148Tg + MO1-col12a1a + MO1-col12a1b
Condition:	perforation: spinal cord
Knockdown Reagents:	MO1-col12a1a MO1-col12a1b
Observed In:	regenerating tissue axon spinal cord axon regeneration swimming
Stage:	Day 5

Phenotype Detail

Acknowledgments

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