Fig. 1
- ID
- ZDB-FIG-161122-2
- Publication
- Bhattarai et al., 2016 - IL4/STAT6 Signaling Activates Neural Stem Cell Proliferation and Neurogenesis upon Amyloid-β42 Aggregation in Adult Zebrafish Brain
- Other Figures
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Aβ42 Injection Paradigm and Aggregation-Dependent Phenotypes in Adult Zebrafish Brain (A) Immunohistochemistry (IHC) for Aβ42 in control brains at 1, 3, and 14 days post-injection (dpi) of PBS. Right panels: green channel. (B-D) IHC for Aβ42 in brains injected with uncoupled Aβ42 (B), R9-Aβ42 (C), or TR-Aβ42 (D). (E) Quantification for green fluorescence intensity as a measure of amyloid deposition. (F) CD spectra for all Aβ42 versions in buffer (PBS). Aβ42 peptides show β sheet formation. (G-H') Electron micrographs of TR-alone- (G) and TR-Aβ42-injected (H) brains. Blue, nucleus; green, amyloid. (G' and H') Higher magnification of perinuclear regions with Aβ42. (I and I') IHC for Aβ42 in control (I) and TR-Aβ42-injected (I’) brains. Green and DAPI are shown separately. (J and J') TUNEL for control (J) and TR-Aβ42-injected (J') brains. Insets: single channels of a close-up region. (K and K') HuC/D and L-Plastin in control (K) and TR-Aβ42-injected (K') brains. (L and L') Synaptophysin in control (L) and TR-Aβ42-injected (L') brains. (M) Quantification for (J-L'). (N) IHC for HuC/D and TR-Aβ42 coupled to TUNEL staining. (O) Categorization of TUNEL-positive cells according to HuC/D expression and presence of Aβ42. Scale bars, 100 μm. Data are represented as mean ± SD; n = 3 fish. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. See also Figures S1–S3. |
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| Stage: | Adult |
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| Stage: | Adult |