Fig. 1

A?42 Injection Paradigm and Aggregation-Dependent Phenotypes in Adult Zebrafish Brain

(A) Immunohistochemistry (IHC) for A?42 in control brains at 1, 3, and 14 days post-injection (dpi) of PBS. Right panels: green channel.

(B-D) IHC for A?42 in brains injected with uncoupled A?42 (B), R9-A?42 (C), or TR-A?42 (D).

(E) Quantification for green fluorescence intensity as a measure of amyloid deposition.

(F) CD spectra for all A?42 versions in buffer (PBS). A?42 peptides show ? sheet formation.

(G-H') Electron micrographs of TR-alone- (G) and TR-A?42-injected (H) brains. Blue, nucleus; green, amyloid. (G' and H') Higher magnification of perinuclear regions with A?42.

(I and I') IHC for A?42 in control (I) and TR-A?42-injected (I?) brains. Green and DAPI are shown separately.

(J and J') TUNEL for control (J) and TR-A?42-injected (J') brains. Insets: single channels of a close-up region.

(K and K') HuC/D and L-Plastin in control (K) and TR-A?42-injected (K') brains.

(L and L') Synaptophysin in control (L) and TR-A?42-injected (L') brains.

(M) Quantification for (J-L').

(N) IHC for HuC/D and TR-A?42 coupled to TUNEL staining.

(O) Categorization of TUNEL-positive cells according to HuC/D expression and presence of A?42.

Scale bars, 100 ?m. Data are represented as mean ± SD; n = 3 fish. ^?p < 0.05; ^??p < 0.01; ^???p < 0.001. See also Figures S1?S3.