FIGURE

Fig. 2

ID

ZDB-FIG-161122-3

Publication

Bhattarai et al., 2016 - IL4/STAT6 Signaling Activates Neural Stem Cell Proliferation and Neurogenesis upon Amyloid-β42 Aggregation in Adult Zebrafish Brain

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Fig. 2

Aβ42 Induces Stem Cell Proliferation and Neurogenesis in Adult Zebrafish Brain

(A) PCNA, S100β and DAPI in control brains. Insets: individual channels.

(A') High-magnification of a double-positive cell.

(B) PCNA, S100β, and DAPI in TR-Aβ42-injected brains.

(B') High-magnification of a double positive cell.

(D) Neurogenesis assay.

(E and F) Acetylated tubulin and BrdU in control (E) and TR-Aβ42-injected (F) brains. Insets: single channels.

(G) Number and migration distance of newborn neurons.

(H and I) HuC/D and BrdU in control (H) and TR-Aβ42-injected brains at 2 weeks post-injection (wpi).

(J) Quantification of HuC/D-BrdU double-positive cells.

(K) Acetylated tubulin and BrdU in control and TR-Aβ42-injected brains at 3 months after injection. Insets: examples of newborn neurons.

(L) Quantification of acetylated tubulin and BrdU double-positive cells per section.

Scale bars, 50 μm (A and B) and 20 μm (E, F, H, I, and K). Data are represented as mean ± SD; n = 3 fish. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001. See also Figures S1 and S3 and Table S2.

Expression Data

Antibodies:	Ab1-elavl Ab1-s100 Ab1-tuba Ab2-pcna
Fish:	WT
Condition:	chemical treatment by injection: amyloid-beta polypeptide 42
Anatomical Terms:	brain brain neuron radial glial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by injection: amyloid-beta polypeptide 42
Observed In:	brain cell population proliferation brain neurogenesis radial glial cell cell population proliferation
Stage:	Adult

Phenotype Detail

Acknowledgments

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