FIGURE

Fig. 6

ID

ZDB-FIG-150615-6

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 6

Connecting cilia are reduced in pard3 morphants. (A–C) Transverse cryosections were stained for Ift88 (left, red) and acetylated tubulin (middle, green) to label cilia in 5 dpf retinas. Merged images are shown in right panels. Ift88 localizes to the connecting cilia emanating from the apical surface of photoreceptors (arrows). Staining is also seen in connecting cilia of UV cones (arrowheads), which are tiered below the other cones. Sections were also counterstained with DAPI (blue). ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 10 μm.

Expression Data

Gene:	ift88
Antibodies:	Ab1-ift88 Ab1-tuba
Fish:	AB/EKW AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Anatomical Term:	eye photoreceptor cell photoreceptor connecting cilium
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Observed In:	eye photoreceptor cell eye photoreceptor cell photoreceptor connecting cilium
Stage:	Day 5

Phenotype Detail

Acknowledgments

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