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Left-right asymmetry requires pard3. (A) In situ hybridization for southpaw (spaw) expression in the lateral plate mesoderm in 18–20 somite stage embryos. pard3 morphants show left-sided, right-sided, bilateral, or an absence of spaw expression. (B) Graphical summary from three independent experiments showing the percentage of control and morphant embryos with left-sided (L), right-sided (R), bilateral (B), or absence (A) spaw expression. The number (n) of embryos analyzed is given next to each set of bars. (C) KV cilia in a wild-type and pard3 morphant. (D) Quantification of KV length across the longest axis. (E) Quantification of KV cilia number. (F) Quantification of KV cilia length. Bars show mean ± standard deviation (*p≤0.05; ***p≤0.0001) Scale bar = 20 μm.
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