FIGURE

Fig. 1

ID

ZDB-FIG-150615-1

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 1

Spatial distribution of cell junction proteins and cilia in 5 dpf zebrafish. (A–E) Left-most panels show transverse cryosections stained with phalloidin (red) to label actin in the adherens junctions at the outer limiting membrane (OLM) and the outer plexiform layer (OPL). The middle panels show staining for determinants of apico-basal polarity (green), while the right-most panels show the merged images. (A1–A3) PrkCi/z immunoreactivity. (B1–B3) Pard3 immunoreactivity localizes to the OPL (arrow) and at cell junctions (arrowhead). (C1–C3) ZO-1 staining at the OLM. (D1–D3) Ift88 immunoreactivity stains cilia. (E1–E3) Acetylated tubulin immunoreactivity was seen in cilia and neural processes throughout the inner nuclear layer and plexiform layers. (F1–F3) Pard3 staining (red) was seen at the OLM (arrowhead), but did not colocalize with acetylated tubulin signal in the cilia (arrows). All sections were counterstained with DAPI (blue). Scale bar = 10 μm.

Expression Data

Genes:	ift88 pard3ab
Antibodies:	Ab1-ift88 Ab1-prkcz Ab1-tjp1 Ab1-tuba Ab3-pard3
Fish:	AB/EKW
Anatomical Terms:	eye photoreceptor cell photoreceptor connecting cilium outer limiting membrane retinal outer plexiform layer retinal photoreceptor layer apical region retinal photoreceptor layer cell junction
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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