FIGURE

Fig. 5

ID

ZDB-FIG-150615-5

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 5

Morpholino suppression of Pard3 causes variable cyclopia and loss of photoreceptor outer segments. (A–C) Transverse plastic sections of 4 dpf larvae heads from wild-type and pard3 morphants show variable degrees of cyclopia but normal lamination. (D–F) Higher magnification of the boxed areas in A–C revealed the photoreceptor outer segments in wild-type retinas (Panel D, arrows), which were missing in pard3 morphants. Morphants with fused eyes also exhibited patchy pigmentation in the RPE (Panel E, arrowheads). (G–H) Transmission electron microscopy of wild-type and pard3 morphant retinas showed truncation of photoreceptor outer segments. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bar = 50 μm (A–C); 10 μm (D–F); 1 μm (G–H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab
Knockdown Reagent:	MO1-pard3ab
Observed In:	eye photoreceptor cell eye photoreceptor cell photoreceptor outer segment retina layer formation retinal pigmented epithelium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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