FIGURE

Fig. 3

ID

ZDB-FIG-150416-23

Publication

Wen et al., 2015 - Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis

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Fig. 3

Persistence of laminin expression at the choroid fissure and altered proximo-distal patterning of the optic vesicle in sox4 morphants. (A–D′) Laminin immunostaining on control and sox4 morphant embryos at 48 and 72 hpf. Representative images from 15–20 individuals analyzed for each group are shown. (E–H) Double FISH for pax2a and pax6a at 18 and 48 hpf. (I) qPCR analysis revealed a 1.69-fold increase in pax2a expression and a 2.0-fold decrease in pax6a expression in sox4 morphant heads at 18 hpf (Student′s t-test, P<0.05). All scale bars equal 50 µm. Asterisks in A′ and C′ indicate the closing or fused choroid fissure in control morphants; brackets in B′ and D′ indicate the open choroid fissure in sox4 morphants.

Expression Data

Genes:	pax2a pax6a
Fish:	WT WT + MO2-sox4a + MO3-sox4b + MO4-tp53
Knockdown Reagents:	MO2-sox4a MO3-sox4b MO4-tp53
Anatomical Terms:	optic stalk optic vesicle retina
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-sox4a + MO3-sox4b + MO4-tp53
Knockdown Reagents:	MO2-sox4a MO3-sox4b MO4-tp53
Observed In:	closure of optic fissure optic fissure
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 399(1), Wen, W., Pillai-Kastoori, L., Wilson, S.G., Morris, A.C., Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis, 139-53, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.