FIGURE

Fig. 7

ID

ZDB-FIG-150416-27

Publication

Wen et al., 2015 - Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Overexpression of sox4 inhibits Hh ligand expression and causes cyclopia. (A–F) Representative images of embryos injected with control tdTomato or sox4 mRNA. (A-B) Representative images of the head were taken at 72 hpf from the ventral side. (C-F) Lateral views of body showing the somites. Insets in (C) and (D) were enlarged in (E) and (F). (G) Quantification of the percentage of cyclopia in embryos injected with different combinations of control and sox4 mRNA. 1.0 ng/embryo Td-tomato: 0.76±1.31%, n=1/108; 0.2 ng sox4a/b+0.6 ng Td-tomato/embryo: 9.35±1.17%, n=13/139; 0.3 ng sox4a/b+0.4 ng Td-tomato/embryo: 17.03±3.69%, n=11/67; 0.4 ng sox4a/b+0.2 ng Td-tomato/embryo: 25.56±2.20%, n=25/97. (H) qPCR analysis of ihhb and shha expression in control and sox4 overexpressing heads at 18 and 24 hpf. In sox4 mRNA injected embryos, ihhb expression was significantly decreased by 1.61-fold at 18 hpf (Studentós t-test, P<0.05) and 1.51-fold at 24 hpf (Studentós t-test, P=0.057). Shha expression was also significantly reduced following sox4 mRNA injection at both 18 and 24 hpf (by 1.82-fold and 3.57-fold, respectively; Student′s t-test, P<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 399(1), Wen, W., Pillai-Kastoori, L., Wilson, S.G., Morris, A.C., Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis, 139-53, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.