ZFIN Image: Wen et al., 2015, Fig. 3

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Fig. 3

Persistence of laminin expression at the choroid fissure and altered proximo-distal patterning of the optic vesicle in sox4 morphants. (A–D′) Laminin immunostaining on control and sox4 morphant embryos at 48 and 72 hpf. Representative images from 15–20 individuals analyzed for each group are shown. (E–H) Double FISH for pax2a and pax6a at 18 and 48 hpf. (I) qPCR analysis revealed a 1.69-fold increase in pax2a expression and a 2.0-fold decrease in pax6a expression in sox4 morphant heads at 18 hpf (Student′s t-test, P<0.05). All scale bars equal 50 µm. Asterisks in A′ and C′ indicate the closing or fused choroid fissure in control morphants; brackets in B′ and D′ indicate the open choroid fissure in sox4 morphants.

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Acknowledgments

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Reprinted from Developmental Biology, 399(1), Wen, W., Pillai-Kastoori, L., Wilson, S.G., Morris, A.C., Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis, 139-53, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.