FIGURE

Fig. 5

ID

ZDB-FIG-150416-25

Publication

Wen et al., 2015 - Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis

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Fig. 5

Hh signaling is elevated in Sox4-deficent zebrafish. (A–D′) Representative eye and whole body images of vehicle-treated and cyclopamine-treated sox4 morphants at 24 and 48 hpf. (E) Quantification of the percentage of coloboma in vehicle- and cyclopamine-treated control and sox4 morphants (control morphants+vehicle: 1.14±0.96% coloboma, n=2/175; control morphants+cyclopamine: 0.49±0.79% coloboma, n=1/205; sox4 morphants+vehicle: 57.78±2.28% coloboma, n=104/180; sox4 morphants+cyclopamine: 15.87±2.20% coloboma, n=26/165; Fisherós exact test, P<0.0001). (F–I) Representative eye and body lateral images (F–G′) and transverse sections (H, I) of ptch2:EGFP(+/) transgenic embryos injected with control (F-F′, H) or sox4 MO (G-G′, I) at 12 hpf. (J) qPCR analysis of ptch2 expression in embryos injected with control or sox4 MO at 8, 10, and 12 hpf. Relative transcript abundance was normalized to level of atp5h (three biological replicates; Student′s t-test, P<0.01).

Expression Data

Gene:	EGFP
Fish:	umz24Tg umz24Tg + MO2-sox4a + MO3-sox4b + MO4-tp53
Knockdown Reagents:	MO2-sox4a MO3-sox4b MO4-tp53
Anatomical Term:	forebrain neural rod ventral region
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-sox4a + MO3-sox4b + MO4-tp53 umz24Tg + MO2-sox4a + MO3-sox4b + MO4-tp53
Condition:	chemical treatment: Cyclopamine
Knockdown Reagents:	MO2-sox4a MO3-sox4b MO4-tp53
Observed In:	closure of optic fissure optic fissure smoothened signaling pathway
Stage Range:	5-9 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 399(1), Wen, W., Pillai-Kastoori, L., Wilson, S.G., Morris, A.C., Sox4 regulates choroid fissure closure by limiting hedgehog signaling during ocular morphogenesis, 139-53, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.