FIGURE

Fig. S21

ID

ZDB-FIG-141007-62

Publication

Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S21

To assess the effect of the inhibitor on embryo development, dose response experiments were performed. Embryos treated with 1 μg/mL (+)-JQ1 from 6 hpf died by 24 hpf. The same concentration of the inactive enantiomer ()-JQ1 did not affect the development of embryos. Incubation in lower concentration of (+)-JQ1 (0.5 μg/ml) led to aberrant development, i.e. tail malformation and heart edema. The lowest tested dose, 0.25 μg/ml, did not affect the development of the embryos. To avoid early embryonic lethality (+)-JQ1 inhibitor was added at 24 hpf. The embryos exhibited overall normal development even at the higher concentration (1 μg/ml) of (+)-JQ1. Representative images of embryo morphology are shown, taken at 24 hpf, 48 hpf and 72 hpf. All the embryos are positioned with anterior to the left and dorsal to the top.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.