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Fig. S21
To assess the effect of the inhibitor on embryo development, dose response experiments were performed. Embryos treated with 1 μg/mL (+)-JQ1 from 6 hpf died by 24 hpf. The same concentration of the inactive enantiomer ()-JQ1 did not affect the development of embryos. Incubation in lower concentration of (+)-JQ1 (0.5 μg/ml) led to aberrant development, i.e. tail malformation and heart edema. The lowest tested dose, 0.25 μg/ml, did not affect the development of the embryos. To avoid early embryonic lethality (+)-JQ1 inhibitor was added at 24 hpf. The embryos exhibited overall normal development even at the higher concentration (1 μg/ml) of (+)-JQ1. Representative images of embryo morphology are shown, taken at 24 hpf, 48 hpf and 72 hpf. All the embryos are positioned with anterior to the left and dorsal to the top.