FIGURE

Fig. S15

ID

ZDB-FIG-141007-57

Publication

Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis

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Fig. S15

Individual neutrophils may be detected with Sudan Black staining. The number of neutrophils in MO-injected embryos (n_embryos = 15) in CHT was counted at 3 dpf. Knock-down of akap10, brd3a, brf1b, kif1b, waspla or wasplb caused no phenotype. A mild decrease (20–50%) in the number of neutrophils was observed for brf1a, kalrn2, pdia5, rcor1, satb1 and wdr66 MO injected embryos compared to control and kalrn1, mfn2 and psmd13 MO injected embryos showed a severe (over 50%) depletion of neutrophils when compared to control. Representative images of the CHT region at 3 dpf are shown. All embryos are oriented with anterior to the left and dorsal to the top.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-brf1a WT + MO1-cfap251 WT + MO1-kalrna WT + MO1-kalrnb WT + MO1-pdia5 WT + MO1-psmd13 WT + MO1-satb1b WT + MO2-mfn2 WT + MO2-rcor1
Knockdown Reagents:	MO1-brf1a MO1-cfap251 MO1-kalrna MO1-kalrnb MO1-pdia5 MO1-psmd13 MO1-satb1b MO2-mfn2 MO2-rcor1
Observed In:	blood island neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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