FIGURE

Fig. 5

ID

ZDB-FIG-141007-46

Publication

Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

brd3a is an important regulator of thrombopoiesis.

(A) Live confocal imaging of zebrafish embryos injected with hBRD3-GFP mRNA revealed the nuclear localization of hBRD3 and that it binds to mitotic chromosomes (white arrows). Expression of hBRD3 in brd3a MO injected embryos resulted in a partial rescue of the number of thrombocytes as shown in (B). (C) A graph to illustrate the number of thrombocytes in control, splice brd3a MO and splice brd3a MO plus hBRD3 mRNA injected embryos. Each dot represents the number of thrombocytes in the individual MO-injected embryos with respect to control. A blue horizontal line represents the mean value of the number of thrombocytes for each group of embryos. Student t test, * p = 0.016; n = 17. All embryos are oriented with anterior to the left and dorsal to the top.

Expression Data

Gene:	EGFP
Fish:	la2Tg la2Tg + MO1-brd3a
Knockdown Reagent:	MO1-brd3a
Anatomical Term:	thrombocyte
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la2Tg + MO1-brd3a
Knockdown Reagent:	MO1-brd3a
Observed In:	thrombocyte
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.