ZFIN Image: Bielczyk-Maczyńska et al., 2014, Fig. S15

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Figure Caption

Fig. S15

Individual neutrophils may be detected with Sudan Black staining. The number of neutrophils in MO-injected embryos (n_embryos = 15) in CHT was counted at 3 dpf. Knock-down of akap10, brd3a, brf1b, kif1b, waspla or wasplb caused no phenotype. A mild decrease (20–50%) in the number of neutrophils was observed for brf1a, kalrn2, pdia5, rcor1, satb1 and wdr66 MO injected embryos compared to control and kalrn1, mfn2 and psmd13 MO injected embryos showed a severe (over 50%) depletion of neutrophils when compared to control. Representative images of the CHT region at 3 dpf are shown. All embryos are oriented with anterior to the left and dorsal to the top.

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Phenotype details

Acknowledgments

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