FIGURE

Fig. 1

ID

ZDB-FIG-140414-15

Publication

Johnson et al., 2014 - Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube

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Fig. 1

Characterization of kif11 expression in radial glia and its effect on radial glial soma localization. (A–D) kif11 siblings were grown to 30 hpf and labeled for radial glia (Gfap) and acetylated Tubulin (AT) to identify glial and axon defects. In wild type siblings a small population of Gfap+ radial glial somas can be visualized at the luminal surface in a maximum intensity projection of optical sections along the parasagittal plane that encompass the ventricular zone (A) as well as in cross section of the neural tube (B). An increase in the number of radial glial somas can be seen the neural tube of kif11^-/- mutants (C,D). (E) Quantification of Gfap+ somas counted from maximum intensity projections of parasagittal optical slices within the neural tube and the total number of nuclei in transverse cross sections of the neural tube showed an 8-fold increase in the number of Gfap+ cell bodies in kif11^-/- mutants, but no change in the number of total nuclei between siblings. (F) RT-PCR analysis of kif11 expression between pooled wild type/heterozygote siblings and kif11^-/- at 48 hpf. β-actin was used as a loading control. (G–M) Wild type kif11 mRNA was present throughout the forebrain (G, lateral; H, dorsal) and trunk (I, J–M) at 27 hpf, concentrated at the luminal surface of the VZ (J,K) and into the floor plate (J,K, insets). (K–M) Fluorescent kif11 detection (K) within a Tg(gfap:EGFP) embryo (L) shows that kif11 mRNA is present down the length of the ventricular zone and colocalizes with cells expressing GFP associated with the lumen of the VZ (M).

Expression Data

Genes:	GFP kif11
Antibody:	zrf-1
Fish:	WT kif11^{hi3112aTg/hi3112aTg} mi2001Tg
Anatomical Terms:	floor plate forebrain radial glial cell trunk ventricular zone
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kif11^{hi3112aTg/hi3112aTg}
Observed In:	neural tube radial glial cell
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.