FIGURE

Fig. 3

ID

ZDB-FIG-140414-17

Publication

Johnson et al., 2014 - Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Loss of Kif11 causes monoaster spindle formation in radial glial cells during division. kif11 siblings and wild type embryos treated with either STLC or DMSO control from 5 to 30 hpf were labeled for radial glia (Gfap), microtubules (α-Tubulin) (microtubules), and histones (Hoechst stain) to identify mitotic spindle phenotypes. (A) kif11 wild type siblings and (I) vehicle control (DMSO) treated embryos exhibit normal spindles in M-phase (enlarged in B–D and J–L, respectively) while (E) kif11^-/- mutants and (M) STLC treated embryos display monoastral spindles characteristic of mitotic arrest (enlarged in F–H and N–P, respectively). White dots designate presumptive locations of centrosomes. For greater clarity Hoechst is displayed as a greyscale image in the enlargements (D, H, L, P).

Expression Data

Antibody:	zrf-1
Fish:	WT kif11^{hi3112aTg/hi3112aTg}
Condition:	chemical treatment: pharmaceutical
Anatomical Term:	radial glial cell
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT kif11^{hi3112aTg/hi3112aTg}
Condition:	chemical treatment: pharmaceutical
Observed In:	radial glial cell radial glial cell aster
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.