FIGURE

Fig. 5

ID

ZDB-FIG-140414-18

Publication

Johnson et al., 2014 - Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube

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Fig. 5

kif11 mutants have inversely proportional changes to the number of cells dying and proliferating. (A,B) Lateral view of the 25 hpf neural tube labeled for anti-activated Caspase-3 (green) and anti-Gfap (red) antibodies in wild type (A) and kif11^-/- mutants (B). (C,D) Lateral view of the 25 hpf neural tube labeled for anti-BrdU (green) and anti-Gfap (red) antibodies in wild type (C) and kif11^-/- (D). (E) Quantification of cell death (dashed lines) and BrdU incorporation (solid lines). Anti-activated Caspase-3 labeling was increased in kif11^-/- mutants from 15 hpf to 72 hpf (E, dashed blue line), whereas the number of cells in S-phase was reduced from 20 hpf to 36 hpf in kif11^-/- mutants (E, solid black line) as compared with wild type embryos (black lines). (F) Graphical representation of the difference between the number of cells dying (dashed line) or BrdU positive (solid line) between kif11 siblings.

Expression Data

Antibody:	zrf-1
Fish:	WT kif11^{hi3112aTg/hi3112aTg}
Anatomical Term:	radial glial cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kif11^{hi3112aTg/hi3112aTg}
Observed In:	neural tube apoptotic process
Stage Range:	10-13 somites to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.