FIGURE

Fig. 2

ID

ZDB-FIG-140311-7

Publication

Lahvic et al., 2013 - Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Heart jog direction was randomized by hspb7 knockdown. (A) Two splice-blocking morpholinos that target hspb7 both significantly increased right and midline heart jogs at the expense of leftward heart jogs (Χ² test, p<0.001). (B, C) In situ hybridization of myl7 (cmlc2) in embryos injected with hspb7MO1. (B) Left jog and (C) right jog. (D) Live embryo at 26 hpf, injected with control MO. (E) Live embryo at 26 hpf, injected with hspb7MO1, showing characteristic downward curl of the body. (F) The combination of hspb7MO1 (0.25 pmole) and hspb7MO2 (0.17 pmole) prevented heart field fusion when targeted to the whole embryo at 1-4 cell stages. (G-I) In situ hybridization for myl7 (cmlc2). (G) Embryo injected with control MO at 1 cell stage had normal leftward heart jogs; the anterior boundary of the heart is midway across the eye at 26 hpf. (H) Midline heart in embryo injected with hspb7MO1+MO2 at 1 cell stage, showing slowed anterior migration, with the anterior boundary of the heart at the posterior end of the eye. (I) hspb7MO1+MO2 injected embryo, with normal leftward jog but delayed anterior migration and wide heart. (J) hspb7MO1+MO2 injected embryo, with cardia bifida. (K) Yolk injection between 3 and 4 hpf of control MO produced a small number of midline hearts; hspb7MO1 (0.5 pmole) induced midline hearts; hspb7MO1 (0.25 pmole) and hspb7MO2 (0.25 pmole) induced cardia bifida and midline hearts.

Expression Data

Gene:	myl7
Fish:	WT WT + MO3-hspb7 WT + MO3-hspb7 + MO4-hspb7
Knockdown Reagents:	MO3-hspb7 MO4-hspb7
Anatomical Term:	primitive heart tube
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-hspb7 WT + MO3-hspb7 + MO4-hspb7 WT + MO4-hspb7
Knockdown Reagents:	MO3-hspb7 MO4-hspb7
Observed In:	heart jogging heart rudiment whole organism whole organism axis whole organism right side
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.