FIGURE

Fig. 3

ID

ZDB-FIG-140311-8

Publication

Lahvic et al., 2013 - Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Heart jog direction was randomized by hspb12 knockdown (A) Two morpholinos that target hspb12 significantly increased right and midline heart jogs at the expense of leftward jogs compared to control MO (Χ² test; p<0.001 for hspb12MO1 and p<0.05 for hspb12MO2). (B) Yolk injections of hspb12MO1 increased midline hearts but not rightward jogs. Combinations of both hspb12 MOs were lethal. (C) Combined hspb7MO1 and hspb12MO1 (0.25 pmol individually or in combination) injected at the 1-cell stage increased midline hearts and rightward jogs significantly compared to control (Χ² test; all p<0.001). Combined hspb7MO1+hspb12MO1 (0.25 pmol each) gave significantly more abnormal jogs than 0.25 pmol hspb7MO1 alone (Χ² test; p<0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-hspb7 WT + MO3-hspb12 WT + MO4-hspb12 WT + MO3-hspb12 + MO3-hspb7
Knockdown Reagents:	MO3-hspb7 MO3-hspb12 MO4-hspb12
Observed In:	heart jogging heart rudiment whole organism axis whole organism right side
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.