ZFIN Image: Lahvic et al., 2013, Fig. 2

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Fig. 2

Heart jog direction was randomized by hspb7 knockdown. (A) Two splice-blocking morpholinos that target hspb7 both significantly increased right and midline heart jogs at the expense of leftward heart jogs (Χ² test, p<0.001). (B, C) In situ hybridization of myl7 (cmlc2) in embryos injected with hspb7MO1. (B) Left jog and (C) right jog. (D) Live embryo at 26 hpf, injected with control MO. (E) Live embryo at 26 hpf, injected with hspb7MO1, showing characteristic downward curl of the body. (F) The combination of hspb7MO1 (0.25 pmole) and hspb7MO2 (0.17 pmole) prevented heart field fusion when targeted to the whole embryo at 1-4 cell stages. (G-I) In situ hybridization for myl7 (cmlc2). (G) Embryo injected with control MO at 1 cell stage had normal leftward heart jogs; the anterior boundary of the heart is midway across the eye at 26 hpf. (H) Midline heart in embryo injected with hspb7MO1+MO2 at 1 cell stage, showing slowed anterior migration, with the anterior boundary of the heart at the posterior end of the eye. (I) hspb7MO1+MO2 injected embryo, with normal leftward jog but delayed anterior migration and wide heart. (J) hspb7MO1+MO2 injected embryo, with cardia bifida. (K) Yolk injection between 3 and 4 hpf of control MO produced a small number of midline hearts; hspb7MO1 (0.5 pmole) induced midline hearts; hspb7MO1 (0.25 pmole) and hspb7MO2 (0.25 pmole) induced cardia bifida and midline hearts.

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Acknowledgments

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Reprinted from Developmental Biology, 384(2), Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J., Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish, 166-180, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.