FIGURE

Fig. 5

ID
ZDB-FIG-131205-26
Publication
Tsedensodnom et al., 2013 - Ethanol metabolism and oxidative stress are required for unfolded protein response activation and steatosis in zebrafish with alcoholic liver disease
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Ethanol metabolism and ROS are required for secretory pathway stress. (A) Heatmap of qPCR data from liver cDNA measuring the expression of genes involved in the UPR. The fold changes of the median CT values are in supplementary material Table S3. All six clutches are aligned according to the order of the lowest to the highest expression of bip in 350 mM ethanol treatment. (B) Representative images of the tail of Tg(l-fabp:Dbp-EGFP) 120 hpf larvae treated with 0 or 350 mM ethanol or co-treated with AA and 350 mM ethanol for 24 hours. The rectangles in the left panel are magnified in the right panel. Scale bars: 0.2 mm for the left panel and 0.04 mm for the right panel. Note that the curved tail in untreated larvae is an artifact of fixation. (C) Immunoblots of transgenic Tg(l-fabp:Dbp-EGFP) larvae treated as in B using anti-GFP antibody and a non-specific band serving as a loading control. (D,E) Low concentration of ethanol and H2O2 synergize to induce UPR (D) and oxidative stress (E). Heatmaps of qPCR data from the livers of larvae exposed to individual treatments of 100 mM ethanol or 2.1 mM H2O2, or a co-treatment of the two, for 24 hours. The individual CT values are in supplementary material Tables S4 and S5 for D and E, respectively. All six clutches are aligned according to the order of the lowest to the highest expression of bip (D) or sod2 (E) in the co-treatment of 350 mM ethanol and 2.1 mM H2O2.

Expression Data
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: Day 4 to Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.