FIGURE

Fig. 6

ID

ZDB-FIG-131009-26

Publication

Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling

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Fig. 6

Hindbrain patterning in Zic morpholino-injected embryos. mRNA expression of hindbrain segmentation markers hoxa2b/aldh1a2(A, B), krox20/hoxb4a/wnt1(C, D), hoxb1a(E,F), mafba/wnt1(G, H), and egfl6(I-L) were examined in control uninjected (A, C, E, G, I, J) and Zic2a;Zic2b;Zic3MO-injected embryos (B, D, F, H, K, L). We note a reduction in hoxa2b expression in Zic morphant embryos (compare B to A) as well as a thinning of rhombomeres 3 and 5, as labeled by krox20 expression (compare D to C). Expression of hoxb4a is reduced (compare D to C), but other markers of segment identity are overtly normal (E-L). Embryos are shown in dorsal (A-H, J, L) or lateral (I, K) views and are 18 hpf.

Expression Data

Genes:	aldh1a2 egfl6 egr2b hoxa2b hoxb1a hoxb4a mafba wnt1
Fish:	WT WT + MO2-zic2b + MO2-zic3 + MO3-zic2a
Knockdown Reagents:	MO2-zic2b MO2-zic3 MO3-zic2a
Anatomical Terms:	hindbrain rhombomere 3 rhombomere 5
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-zic2b + MO2-zic3 + MO3-zic2a
Knockdown Reagents:	MO2-zic2b MO2-zic3 MO3-zic2a
Observed In:	rhombomere 3 rhombomere 5
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.