FIGURE

Fig. 5

ID

ZDB-FIG-131009-25

Publication

Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling

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Fig. 5

Gene expression changes upon Zic2 knockdown can be rescued by RA treatment and is mimicked by Aldh1a2 knockdown. mRNA in situ analysis of meis3(A-D, I-J), hoxd4a(E-H, K-L) and aldh1a2(M-N). Uninjected embryos were treated with DMSO (A, E) or 5 nm RA (B, F) and compared to Zic2a;Zic2bMO-injected embryos treated with DMSO (C, G) or 5 nm RA (D, H). The decrease in meis3 and hoxd4a observed in Zic morpholino-injected embryos is rescued by treatment with 5 nm RA. Aldh1a2MO-injected embryos (J, L) show a decrease in meis3 and hoxd4a when compared to controls (I, K). In cyp26a1 (giraffe) mutants (N), aldh1a2 levels remain unchanged compared to WT siblings (M). Images are dorsal views of 10.5 hpf (A-L) and 8 hpf (M-N) embryos oriented with anterior to the top.

Expression Data

Genes:	aldh1a2 hoxd4a meis3
Fish:	WT cyp26a1^rw716/rw716 WT + MO2-aldh1a2 WT + MO2-zic2b + MO3-zic2a + MO4-tp53
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagents:	MO2-aldh1a2 MO2-zic2b MO3-zic2a MO4-tp53
Anatomical Terms:	hindbrain neural plate margin
Stage Range:	75%-epiboly to 1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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